Newcastle Laboratories

Phenylalanine and tyrosine

Clinical Background:

Phenylketonuria (PKU) is one of the most common inherited metabolic disorders with an average incidence across the UK of approximately 1 in 10,000 births, although there is geographical variance. P...

Phenylketonuria (PKU) is one of the most common inherited metabolic disorders with an average incidence across the UK of approximately 1 in 10,000 births, although there is geographical variance. PKU is an autosomal recessively inherited disorder of amino acid metabolism caused by a deficiency of the enzyme phenylalanine hydroxylase. This enzyme is required to metabolise phenylalanine to tyrosine, and a deficiency results in the accumulation of phenylalanine and associated metabolites in blood and tissues. The infant brain is sensitive to high phenylalanine levels and if left untreated, patients with PKU develop severe mental retardation, microcephaly and a proportion of patients develop epilepsy. Children with PKU requiring treatment are treated with a phenylalanine restricted diet to reduce the flux through the affected metabolic pathway thereby preventing the accumulation of toxic metabolites. Dietary therapy utilises a phenylalanine-free synthetic amino acid mixture as a substitute for natural protein and requires careful management to ensure appropriate vitamins and trace elements together with small amounts of natural protein are provided. Blood phenylalanine levels must be closely monitored and diet adjusted so that blood phenylalanine levels stay within accepted ranges to enable normal growth and intellectual function.

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Test Details

  • Discipline:

    Biochemistry

    Biochemistry

  • Minimum Volume Adult:

    0.5 mL blood

    0.5 mL blood

  • Minimum Volume Paediatric:

    0.2 mL blood

    0.2 mL blood

  • Special Requirement:

    This test is normally only used for monitoring the management of known diagnosed PKU patients.
    Whole blood only.

    This test is normally only used for monitoring the management of known diagnosed PKU patients.
    Whole blood only.

  • Quality Assurance:

    NEQAS

    NEQAS

  • Reference Ranges:

     

     

    Phe

    Tyr

    Up to 4 days

     40-110

     30-135

    5 days to 6 months

     30-130

     13-200

    7 months to 2 years

     40-140

     20-160

    3 years to 10 years

     20-130

     30-130

    11 ye...

     

     

    Phe

    Tyr

    Up to 4 days

     40-110

     30-135

    5 days to 6 months

     30-130

     13-200

    7 months to 2 years

     40-140

     20-160

    3 years to 10 years

     20-130

     30-130

    11 years to 17 years

     30-115

     25-125

    Adult

     40-100

     20-110

    Units: µmol/L

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  • Other Info:

    User Information Sheet – information for adult patients with PKU who carry out home monitoring of their PKU. This information sheet - "Instructions for taking blood samples for monitoring PKU" - pr...

    User Information Sheet – information for adult patients with PKU who carry out home monitoring of their PKU. This information sheet - "Instructions for taking blood samples for monitoring PKU" - provides details of the different methods that can be used for monitoring and how to take the samples. Information on where to obtain supplies and detailed information regarding packaging and mailing is included.

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  • Routine Contact Name:

    Duty Biochemist

  • Routine Telephone:

    Freeman: 0191 244 8889

    RVI: 0191 282 9719

    Freeman: 0191 244 8889

    RVI: 0191 282 9719

  • Routine Email:

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Metabolic Clinical Scientist

    Metabolic Clinical Scientist

  • Specialist Telephone:

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

  • Specialist Email:

Availability:

Available during full access hours
Assayed once daily
Site of analysis: RVI

Turn Around:

Within 2 full access days

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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