Newcastle Laboratories

Vascular Endothelial Growth Factor (VEGF) assay

Clinical Background:

VEGF or VEGF-A (also known as Vascular Permeability Factor (VPF)) is a potent mediator of both angiogenesis and vasculogenesis in the foetus and adult. (1-3)
During embryogenesis VEGF regulates the ...

VEGF or VEGF-A (also known as Vascular Permeability Factor (VPF)) is a potent mediator of both angiogenesis and vasculogenesis in the foetus and adult. (1-3)
During embryogenesis VEGF regulates the proliferation, migration and survival of endothelial cells.
It promotes bone formation through osteoblast and chondroblast recruitment and is also a
monocyte chemoattractant. (3-7)

After birth VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-
vascular endothelial cells.
In adults VEGF functions mainly in wound healing and the female reproductive cycle. (3)

In diseased tissues, VEGF promotes vascular permeability and is thought to contribute to tumour
metastasis by promoting both extravasation and tumour angiogenesis. (8, 9)

Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid
arthritis (RA), multiple sclerosis (ME) and systemic lupus erythematous (SLE). (10)

VEGF is used to monitor treatment response in POEMS syndrome (a rare paraneoplastic syndrome
due to an underlying plasma cell disorder), the acronym refers to several (but not all) of the
features of the syndrome Polyradiculoneuropathy, Organomegaly, Endocrinopathy, Monoclonal
plasma cell disorder and Skin changes

The assay is a quantitative sandwich enzyme immunoassay technique.

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Test Details

  • Discipline:

    Haematology

    Haematology

  • Special Requirement:

    Serum sample taken into an SST (Gel) tube – blood should be allowed to clot for 30 minutes after collection and the sample should then be centrifuged at 1000g for 15 minutes.
    The serum should be dec...

    Serum sample taken into an SST (Gel) tube – blood should be allowed to clot for 30 minutes after collection and the sample should then be centrifuged at 1000g for 15 minutes.
    The serum should be decanted into a plastic storage tube and assayed immediately or stored frozen at -20°C or below until assay
    If storing locally – please double spin the sample at the stated centrifuge speed and store below -20°C, transport samples on ice to arrive still frozen

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  • Sample Stability:

    After separation serum samples are stable at freezer temperature (< -20°C) for at least 3 months  

    After separation serum samples are stable at freezer temperature (< -20°C) for at least 3 months  

  • Transport Requirements:

    After collection samples should be transported to the laboratory without delay – see sample requirements above for storage details. 

    After collection samples should be transported to the laboratory without delay – see sample requirements above for storage details. 

  • Reference Ranges:

    Serum VEGF 62 – 707 pg/ml

    Serum VEGF 62 – 707 pg/ml

  • Factors Affecting Result:

    Incorrect sample preparation or storage may give unreliable results

    Extremely Lipaemic or grossly haemolysed samples may give inaccurate results and will be rejected

    Incorrect sample preparation or storage may give unreliable results

    Extremely Lipaemic or grossly haemolysed samples may give inaccurate results and will be rejected

  • Referenced Documents:

    1. Leung, D.W. et al. (1989) Science 246:1306
    2. Keck, P.J. et al. (1989) Science 246:1309
    3. Byrne, A.M. et al (2005) J.Cell.Mol.Med.9:777
    4. Robinson, C.J. and S.E. Stringer (2001) J.Cell.Sci. 114:8...

    1. Leung, D.W. et al. (1989) Science 246:1306
    2. Keck, P.J. et al. (1989) Science 246:1309
    3. Byrne, A.M. et al (2005) J.Cell.Mol.Med.9:777
    4. Robinson, C.J. and S.E. Stringer (2001) J.Cell.Sci. 114:853
    5. Dai, J. and A.B.Rabie (2007) J.Dent.Res. 86:937
    6. Breier, G. (2000) Semin. Thromb. Hemost. 26:553
    7. Barleon, B. et al. (1996) Blood 87:3336
    8. Weis, S.M and D.A. Cheresh (2005) Nature 437:497
    9. Thurston, G (2002) J. Anat. 200:575
    10. Carvalho, J.F. et al (2007) J. Clin. Immunol. 27:246

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  • Specialist Contact Name:

    Haematology/Haemostasis Health Care Scientist

    Haematology/Haemostasis Health Care Scientist

  • Specialist Telephone:

    0191 213 9758

    0191 213 9758

Turn Around:

The assay is run in batches, normally every month

Send To:

Haematology Special assays laboratory
Blood Sciences
Freeman Hospital
Freeman Road
High Heaton
Newcastle upon Tyne
NE7 7DN

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