Newcastle Laboratories

Transfusion Reaction Investigation

Clinical Background:

Following the administration of a blood or blood component an unexpected reaction occurs. Transfusion of blood and blood products can be clinically beneficial and life-saving, however it also comes...

Following the administration of a blood or blood component an unexpected reaction occurs. Transfusion of blood and blood products can be clinically beneficial and life-saving, however it also comes with the risk of a transfusion reaction. All reactions are reportable to SABRE (SHOT and or MHRA). 

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Test Details

  • Discipline:

    Transfusion

    Transfusion

  • Specimen Container Adult:

    6mL K2 EDTA

    6mL K2 EDTA

  • Specimen Container Paediatric:

    6mL K2 EDTA

    6mL K2 EDTA

  • Minimum Volume Adult:

    6mL

    6mL

  • Minimum Volume Paediatric:

    2mL

    2mL

  • Special Requirement:

    Samples associated with a transfusion reaction investigation can be ordered via the EPR PowerChart and taken with the BloodTrack bedside labelling system and requested via PowerChart. Contact the B...

    Samples associated with a transfusion reaction investigation can be ordered via the EPR PowerChart and taken with the BloodTrack bedside labelling system and requested via PowerChart. Contact the Blood Transfusion Laboratory and Haematology SpR (and Transfusion Practitioner during routine hours) IMMEDIATELY if a transfusion reaction is suspected. ALL transfused or partially transfused products associated with reaction MUST be returned to the Blood Transfusion Laboratory. The giving sets MUST be left in the products. Samples should also be taken for the following tests and sent to the appropriate laboratory: FBC, U&E, LFT, blood cultures  

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  • Sample Stability:

    Short term storage: 24 hours at room temperature.
    Long term storage: 7 days at 4-6°C

    Short term storage: 24 hours at room temperature.
    Long term storage: 7 days at 4-6°C

  • Transport Requirements:

    Sample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot...

    Sample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot or cold conditions prior to testing.

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  • Add On Test:

    Request for add on must be discussed directly with the Transfusion laboratory.
    FH: 0191 2237849
    RVI: 0191 2824435

    Request for add on must be discussed directly with the Transfusion laboratory.
    FH: 0191 2237849
    RVI: 0191 2824435

  • Interpretation:

    A serological positive reaction will demonstrate a specific antibody detected serologically and subsequent transfusion requirements will need antigen negative units compatible with the antibody det...

    A serological positive reaction will demonstrate a specific antibody detected serologically and subsequent transfusion requirements will need antigen negative units compatible with the antibody detected. Clinical advice for patient management will be given to the patient's clinical team by the Transfusion Practitioner, Haematology SpR or Haematology Consultant.
    A serological negative reaction informs the clinician that the reaction was not due to a specific antibody causing a haemolytic reaction. However the reaction could still be due to the component and clinical advice will be given to the patient's clinical team by the Transfusion Practitioner, Haematology SpR or Haematology Consultant to the nature of that reaction and future management.

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  • Factors Affecting Result:

    Insufficient or clotted samples, incorrect specimen type received, failure to return blood bag units, units returned to laboratory without giving sets in situ, failure to send the appropriate speci...

    Insufficient or clotted samples, incorrect specimen type received, failure to return blood bag units, units returned to laboratory without giving sets in situ, failure to send the appropriate specimen to all departments as required.

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  • Other Info:

    To investigate all causes of transfusion reactions. Specimens must be sent with a Transfusion reaction investigation request form (obtain from intranet) completed by medical staff. Samples for FBC,...

    To investigate all causes of transfusion reactions. Specimens must be sent with a Transfusion reaction investigation request form (obtain from intranet) completed by medical staff. Samples for FBC,U&Es,LFTs and blood cultures should also be sent.

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  • Routine Contact Name:

    Transfusion Practitioner team
    FH Transfusion Laboratory Section Leads
    RVI Transfusion Laboratory Section Leads

  • Routine Telephone:

    Transfusion Practitioners: 0191 (24)48853 / (91) 77509
    FH Section Leads: 0191 (21) 37492
    RVI Section Leads: 0191 (28) 24768

    Transfusion Practitioners: 0191 (24)48853 / (91) 77509
    FH Section Leads: 0191 (21) 37492
    RVI Section Leads: 0191 (28) 24768

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Transfusion Manager

    Transfusion Manager

  • Specialist Telephone:

    0192 213 9169

    0192 213 9169

Availability:

24/7

Turn Around:

Inhouse investigations - 4 hours
There will be a delay if the blood component is referred to NHSBT Microbiology laboratory for bacterial contamination investigation.

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