Sickle Solubility Test
Clinical Background:
Haemoglobin S (HbS) or sickle cell haemoglobin, results from an autosomal recessive mutation to the beta globin gene. Red cells containing HbS have a reduced deformability, under conditions of Hypo...
Haemoglobin S (HbS) or sickle cell haemoglobin, results from an autosomal recessive mutation to the beta globin gene. Red cells containing HbS have a reduced deformability, under conditions of Hypoxia they form the so-called sickle shape and are easily destroyed resulting in occlusion of the microcurculation and a chronic haemolyitic anaemia. Heterozygotes have sickle cell trait, homozygotes have sickle cell disease. HbS may be found in conjunction with other Haemoglobin mutations and compound heterozygotes may display the features of homozygous disease or be asymptomatic depending on the combination. The screeing test will detect the presence of HbS in a sample, it will not distunguish between HbAS (heterozygote) and HbSS (homozgote), positive samples are sent for Haemoglobin Quantitation. The test is used as a quick pre-op screening test in patients with a family history of HbS or if the patient is from a region with a high prevelance of the mutation (Africa, Middle East, Mediterranean and India) as general anaesthesia presents a significant risk to paitents with this mutation
Test Details
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Discipline:
Protein laboratory
Protein laboratory
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Specimen Container Adult:
Purple EDTA 4ml14 days if stored between 2-8 degrees
Purple EDTA 4ml14 days if stored between 2-8 degrees
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Specimen Container Paediatric:
Purple EDTA
Purple EDTA
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Minimum Volume Adult:
2.0mL
2.0mL
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Minimum Volume Paediatric:
1.0mL
1.0mL
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Special Requirement:
Ensure adequate mixing.
Ensure adequate mixing.
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Sample Stability:
14 days if stored between 2-8 degrees
14 days if stored between 2-8 degrees
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Transport Requirements:
No Special transport requirements
No Special transport requirements
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Freq Analysis:
Routine testing completed daily Monday to Friday, Urgent testing out of hours completed on request
Routine testing completed daily Monday to Friday, Urgent testing out of hours completed on request
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Add On Test:
Phone EXT 24766 during routine house and EXT 25819 out of hours
All urgent add ons via telephone must be confirmed via email to the appropriate email address
tnu-tr.bloodsciencesadditions@nhs.net (...
Read MorePhone EXT 24766 during routine house and EXT 25819 out of hours
All urgent add ons via telephone must be confirmed via email to the appropriate email address
tnu-tr.bloodsciencesadditions@nhs.net (internal)
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Quality Assurance:
The lab participates in the UKNEQAS Abnormal Haemoglobins EQA scheme
The lab participates in the UKNEQAS Abnormal Haemoglobins EQA scheme
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Interpretation:
Positive – Suggest Haemoglobin S present
Negative – Suggest Haemoglobin S not present.
All results require confirmation via High Performance Liquid Chromatography via a Haemoglobinopathy screen. Du...
Read MorePositive – Suggest Haemoglobin S present
Negative – Suggest Haemoglobin S not present.
All results require confirmation via High Performance Liquid Chromatography via a Haemoglobinopathy screen. During routine hours, this will be completed by the Protein laboratory. If confirmation and haemoglobin S quantitation required urgently out of hours this must be discussed with the Biochemistry Biomedical Scientist on shift EXT 24305/24041.
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Factors Affecting Result:
Patient age – Testing not appropriate for patients under 6 month. Please refer to patient’s Newborn screening result if confirmation of sickle status required.
Post transfusion – Testing not approp...
Read MorePatient age – Testing not appropriate for patients under 6 month. Please refer to patient’s Newborn screening result if confirmation of sickle status required.
Post transfusion – Testing not appropriate if patient is post transfusion. This may give a false negative result. Discuss with laboratory if clinical concern.
Testing should not be completed on clotted sample or haemolysed sample. A repeat sample will be requested.
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Other Info:
N/A
N/A
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Routine Contact Name:
Proteins Lab
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Routine Telephone:
0191 282 4766
0191 282 4766
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Routine Email:
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Specialist Test:
Yes
Yes