Rh Phenotype
Clinical Background:
Phenotype investigations as required to determine the antigens carried on a red blood cell (RBC). The "phenotype" of any blood group refers to which antigens are serologically detectable on the RBC...
Phenotype investigations as required to determine the antigens carried on a red blood cell (RBC). The "phenotype" of any blood group refers to which antigens are serologically detectable on the RBC, and usually (though not always) corresponds to the genes that the patient carries. The test procedure is based on the principle of agglutination. Normal human red cell, processing antigens, will agglutinate in the presence of antibody directed toward the antigen. Rh phenotyping tests for the C, E, c, e and K antigens.
Test Details
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Discipline:
Transfusion
Transfusion
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Specimen Container Adult:
6mL K2 EDTA
1mL paediatric K2 EDTA or 4mL K2 EDTA can be used in exceptional circumstances.6mL K2 EDTA
1mL paediatric K2 EDTA or 4mL K2 EDTA can be used in exceptional circumstances. -
Specimen Container Paediatric:
1mL K2 EDTA or 6mL K2 EDTA
1mL K2 EDTA or 6mL K2 EDTA
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Minimum Volume Adult:
6mL
6mL
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Minimum Volume Paediatric:
1mL
1mL
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Special Requirement:
The Rh phenotype test is not available for order by the Clinical area. The test is performed by the laboratory as part of serological investigations.
The Rh phenotype test is not available for order by the Clinical area. The test is performed by the laboratory as part of serological investigations.
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Sample Stability:
Short term storage: 24 hours at room temperature.
Long term storage: 7 days at 4-6°CShort term storage: 24 hours at room temperature.
Long term storage: 7 days at 4-6°C -
Transport Requirements:
Sample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot...
Read MoreSample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot or cold conditions prior to testing.
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Add On Test:
Request for add on must be discussed directly with the Transfusion laboratory.
FH: 0191 2237849
RVI: 0191 2824435Request for add on must be discussed directly with the Transfusion laboratory.
FH: 0191 2237849
RVI: 0191 2824435 -
Interpretation:
The presence or absence of antigens is identified by agglutination. Agglutination occurs when the red cells are cross-linked by the binding of either IgG or IgM antibody molecules. The blood group ...
Read MoreThe presence or absence of antigens is identified by agglutination. Agglutination occurs when the red cells are cross-linked by the binding of either IgG or IgM antibody molecules. The blood group antigens reported will show whether the patient is positive (demonstrates the antigen) or negative for the antigen type.
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Factors Affecting Result:
Incorrect specimen type received, clotted, haemolysed, icteric or lipaemic samples. Clotted samples may interfere with instrument pipetting and must not be used. Fibrin or particulate matter, clots...
Read MoreIncorrect specimen type received, clotted, haemolysed, icteric or lipaemic samples. Clotted samples may interfere with instrument pipetting and must not be used. Fibrin or particulate matter, clots, bubbles or scratches on the test cassette can interfere with reaction interpretations.
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Routine Contact Name:
FH Section Leads
RVI Section Leads -
Routine Telephone:
Freeman: 0191 213 7849
RVI: 0191 282 4335Freeman: 0191 213 7849
RVI: 0191 282 4335 -
Specialist Test:
Yes
Yes
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Specialist Contact Name:
Transfusion manager
Transfusion manager
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Specialist Telephone:
0192 213 9169
0192 213 9169