Newcastle Laboratories

Respiratory Viral PCR

Clinical Background:

This is qualitative PCR assay used to detect DNA or RNA from respiratory viruses. Standard testing for most patients is an ‘Influenza/RSV screen’. This includes tests for Influenza A, Influenza B a...

This is qualitative PCR assay used to detect DNA or RNA from respiratory viruses. Standard testing for most patients is an ‘Influenza/RSV screen’. This includes tests for Influenza A, Influenza B and RSV. For patients who are immunocompromised or require intensive case an ‘Extended viral respiratory screen’ can be ordered if indicated (link for more information). The extended test can additionally detect Parainfluenzavirus 1 - 4, Metapneumovirus, Adenovirus and Rhinovirus. Where upper respiratory samples are negative and the patient has predominantly lower respiratory symptoms, consideration should be given to sending an induced sputum or BAL. It should be noted that PCR may be positive for several weeks. The detection of viral DNA or RNA does not necessarily equate with ongoing viral replication and infectious virus.

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Test Details

  • Discipline:

    Microbiology

    Microbiology

  • Specimen Container Adult:

    Sputum, BAL, ETS, Nasal, Throat swab or other respiratory sample in sterile universal or viral swab

    Sputum, BAL, ETS, Nasal, Throat swab or other respiratory sample in sterile universal or viral swab

  • Specimen Container Paediatric:

    Sputum, BAL, ETS, Nasal, Throat swab or other respiratory sample in sterile universal or viral swab

    Sputum, BAL, ETS, Nasal, Throat swab or other respiratory sample in sterile universal or viral swab

  • Minimum Volume Adult:

    5 ml

    5 ml

  • Minimum Volume Paediatric:

    1 ml

    1 ml

  • Special Requirement:

    None noted. Send to laboratory as quickly as possible. If delays likely, refrigerate at 2 to 8 °C

    None noted. Send to laboratory as quickly as possible. If delays likely, refrigerate at 2 to 8 °C

  • Sample Stability:

    Stable at fridge/ambient temperature for 24 hours

    Stable at fridge/ambient temperature for 24 hours

  • Transport Requirements:

    Standard

    Standard

  • Add On Test:

    Possible up to 5 days

    Possible up to 5 days

  • Quality Assurance:

    QCMD, PHE proficiency scheme

    QCMD, PHE proficiency scheme

  • Factors Affecting Result:

    If delays likely, refrigerate at 2 to 10°C

    If delays likely, refrigerate at 2 to 10°C

  • Referenced Documents:

    31.12.14

    31.12.14

  • Other Info:

    Done by Newcastle PHE laboratory

    Done by Newcastle PHE laboratory

Availability:

Performed daily during core hours and on Saturdays

Turn Around:

2 days

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