Newcastle Laboratories

PNH screen

Clinical Background:

Paroxysmal Nocturnal Haemoglobinuria (PNH) is a rare, life threatening, acquired haematopoetic stem cell disorder that affects all blood cell lineages. Somatic mutation of the X-linked phosphatidyl...

Paroxysmal Nocturnal Haemoglobinuria (PNH) is a rare, life threatening, acquired haematopoetic stem cell disorder that affects all blood cell lineages. Somatic mutation of the X-linked phosphatidylinositol glycan complementation Class A (PIG-A) gene leads to partial or absolute deficiency of all proteins linked to the cell membrane by a glycol-phosphatidylinositol (GPI) anchor.  

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Test Details

  • Discipline:

    Flowcytometry

    Flowcytometry

  • Specimen Container Adult:

    Purple top EDTA

    Purple top EDTA

  • Specimen Container Paediatric:

    pink top paediatric EDTA

    pink top paediatric EDTA

  • Minimum Volume Adult:

    0.5ml

    0.5ml

  • Minimum Volume Paediatric:

    0.5ml

    0.5ml

  • Sample Stability:

    If kept at 4 degrees samples can be tested up to 3 days old

    If kept at 4 degrees samples can be tested up to 3 days old

  • Transport Requirements:

    Ambient

    Ambient

  • Freq Analysis:

    6 monthly unless clinically indicated.

    6 monthly unless clinically indicated.

  • Add On Test:

    Use the contact details below to discuss if an add-on is required

    Use the contact details below to discuss if an add-on is required

  • Quality Assurance:

    UKNEQAS PNH

    UKNEQAS PNH

  • Reference Ranges:

    PNH clones should not be present

    Sensitivity for neutrophils and red blood cells is 0.01% 

    PNH clones should not be present

    Sensitivity for neutrophils and red blood cells is 0.01% 

  • Other Info:

    A neutrophil count is required.

    A neutrophil count is required.

  • Routine Contact Name:

    Flow Cytometry Lab

  • Routine Telephone:

    0191 282 5078

    0191 282 5078

  • Routine Email:

  • Specialist Email:

    Use the contact details below to discuss if an add-on is required

    Use the contact details below to discuss if an add-on is required

Availability:

All testing performed at RVI  and available during routine hours, Monday to Friday.

Turn Around:

4 working days

Send To:

Department of Blood Sciences
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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