Newcastle Laboratories

Platelet Aggregation

Clinical Background:

Platelets are responsible for forming the primary haemostatic plug during coagluation and normal platelet function is crucial to this. Platelet function is determined by adding a panel of reagents ...

Platelets are responsible for forming the primary haemostatic plug during coagluation and normal platelet function is crucial to this. Platelet function is determined by adding a panel of reagents (agonists) to a suspension of test platelets and measuring the aggregation response of the platelets to the stumuli. The pattern of aggregation seen with the different agonists allows an examination of several different metabolic pathways in the platelet that are required for normal function

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Test Details

  • Discipline:

    Haemostasis

    Haemostasis

  • Specimen Container Adult:

    Light blue - sodium citrate x 4.

    Light blue - sodium citrate x 4.

  • Specimen Container Paediatric:

    Light blue - sodium citrate x 4.

    Light blue - sodium citrate x 4.

  • Minimum Volume Adult:

    10.8mL

    10.8mL

  • Minimum Volume Paediatric:

    5.4mL

    5.4mL

  • Special Requirement:

    Tubes must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

    Tubes must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

  • Sample Stability:

    2 hrs

    2 hrs

  • Transport Requirements:

    Samples should be transported to lab without delay

    Samples should be transported to lab without delay

  • Freq Analysis:

    On request

    On request

  • Add On Test:

  • Quality Assurance:

    UK NEQAS Blood Coagulation (Pilot scheme)

    UK NEQAS Blood Coagulation (Pilot scheme)

  • Reference Ranges:

    Agonists used include:

    Arachidonic acid

    ADP

    Collagen

    Epinephrine

    Ristocetin

    Results will be interpreted by Haematology medical staff.

    Agonists used include:

    Arachidonic acid

    ADP

    Collagen

    Epinephrine

    Ristocetin

    Results will be interpreted by Haematology medical staff.

  • Factors Affecting Result:

    Time limited test, tests must be completed within 4 hours of venepunture

    Time limited test, tests must be completed within 4 hours of venepunture

  • Other Info:

    A measure of platelet function with known agonists.

    A measure of platelet function with known agonists.

  • Routine Contact Name:

    RVI or Freeman Section Leaders

  • Routine Telephone:

    RVI: 0191 2820332, Freeman: 0191 2231195

    RVI: 0191 2820332, Freeman: 0191 2231195

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Haematology and Haemostasis Healthcare Scientist

    Haematology and Haemostasis Healthcare Scientist

  • Specialist Telephone:

    0191 2139758

    0191 2139758

  • Specialist Email:

    alison.brown93@nhs.net

    alison.brown93@nhs.net

Availability:

Available during routine hours, Monday to Friday, must be pre-arranged.

Turn Around:

Due to the labile nature of platelet function ‘in-vitro’ these assays are completed within 2 hrs, authorisation of results may take longer, due to results being reviewed before final authorisation.

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