Newcastle Laboratories

NK cell Granule Release

Clinical Background:

Useful in the investigation of Familial Haemophagocytic Lymphohistiocytosis (FHLH).

Useful in the investigation of Familial Haemophagocytic Lymphohistiocytosis (FHLH).

Test Details

  • Discipline:

    Immunology

    Immunology

  • Specimen Container Adult:

    2 EDTA Required - 1x Patient & 1x Control

    2 EDTA Required - 1x Patient & 1x Control

  • Specimen Container Paediatric:

    2 EDTA Required - 1x Patient & 1x Control

    2 EDTA Required - 1x Patient & 1x Control

  • Minimum Volume Adult:

    Both EDTA tubes - 4ml

    Both EDTA tubes - 4ml

  • Minimum Volume Paediatric:

    Both EDTA tubes - 4ml

    Both EDTA tubes - 4ml

  • Special Requirement:

    The requesting clinician must arrange for a control sample from a healthy volunteer to arrive at the same time as the patient's sample (please label ‘control’).

    Samples should ideally be tested as...

    The requesting clinician must arrange for a control sample from a healthy volunteer to arrive at the same time as the patient's sample (please label ‘control’).

    Samples should ideally be tested as fresh as possible. Keep at room temperature at all times. Samples should arrive in the laboratory no later than 13:00 to be processed the same day.

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  • Transport Requirements:

    Please sent to Flow Cytometry, Blood Sciences Lab, RVI.

    Please sent to Flow Cytometry, Blood Sciences Lab, RVI.

  • Freq Analysis:

    As required

    As required

  • Add On Test:

    Use the contact details below to discuss if an add-on is required

    Use the contact details below to discuss if an add-on is required

  • Interpretation:

    None

    None

  • Reference Ranges:

    Normal Result: Normal

    Normal Result: Normal

  • Other Info:

    This is performed by stimulation with the K562 line +/- overnight incubation with IL-2 to activate the NK cells. Perforin expression should also be requested as per of FHLH investigation. If both a...

    This is performed by stimulation with the K562 line +/- overnight incubation with IL-2 to activate the NK cells. Perforin expression should also be requested as per of FHLH investigation. If both are normal SAP and XIAP should be excluded in males. This test is only available by prior arrangement with the laboratory (Ext:25078) and discussion with a clinical immunologist (paediatric or adult). Samples should ideally be tested as fresh as possible. Keep at room temperature at all times. Samples should arrive in the laboratory no later than 13:00 to be processed on the same day. 

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  • Routine Contact Name:

    Flow Cytometry Lab

  • Routine Telephone:

    0191 282 5078

    0191 282 5078

  • Routine Email:

Availability:

This test is only available by prior arrangement with the laboratory (Ext: 25078) and discussion with a clinical immunologist (paediatric or adult).

 

This test is not available on a Friday.

Turn Around:

4 Days working days

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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