Newcastle Laboratories

Lipase, Serum

Clinical Background:

Lipases are glycoproteins with a molecular weight of 47 kD produced in the acinar cells of the pancreas. They are defined as triglycerides hydrolases which catalyse the cleavage of triglycerides to...

Lipases are glycoproteins with a molecular weight of 47 kD produced in the acinar cells of the pancreas. They are defined as triglycerides hydrolases which catalyse the cleavage of triglycerides to diglycerides with subsequent formation of glycerol and fatty acids. Any disruption to pancreatic tissue either by inflammation, obstruction or increased permeability of the cells will give rise to an elevated lipase concentration.

As a consequence serum lipase analysis can be used in:-

i) the detection and exclusion of acute pancreatitis (in acute upper quadrant abdominal pain)

ii) chronic (relapsing) pancreatitis

iii) obstruction of the pancreatic duct

iv) detection of pancreatic involvement in abdominal diseases

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Test Details

  • Discipline:

    Biochemistry

    Biochemistry

  • Specimen Container Adult:

    Serum-SST

    Serum-SST

  • Specimen Container Paediatric:

    Serum-Plain tube

    Serum-Plain tube

  • Minimum Volume Adult:

    1 mL blood

    1 mL blood

  • Minimum Volume Paediatric:

    0.5 mL blood

    0.5 mL blood

  • Sample Stability:

    Unseparated:  24 hours

    Separated:  at +15° to +25°C, -7 days

                          at +2° to +8°C,     -7 days

    ...

    Unseparated:  24 hours

    Separated:  at +15° to +25°C, -7 days

                          at +2° to +8°C,     -7 days

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  • Add On Test:

    Possible up to 7 days.

    All urgent add ons via telephone on 0191 282 9719 , and must be confirmed via email to the appropriate email address

    tnu-tr.bloodsciencesadditions@nhs.net (internal)

    tnu-tr.b...

    Possible up to 7 days.

    All urgent add ons via telephone on 0191 282 9719 , and must be confirmed via email to the appropriate email address

    tnu-tr.bloodsciencesadditions@nhs.net (internal)

    tnu-tr.bloodsciencesadditions@nhs.net (external)

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  • Interpretation:

    The determination of lipase activity has a role due to its high specificity and rapid response after onset of pancreatic disease.

    After acute pancreatitis the lipase activity increases within 4-8 h...

    The determination of lipase activity has a role due to its high specificity and rapid response after onset of pancreatic disease.

    After acute pancreatitis the lipase activity increases within 4-8 hours, reaches a peak after 24 hours and decreases after 8-14 days. Activity up to 80 times the upper limit of normal is associated with acute serous inflammation. However, there is no correlation between the lipase activity determined in serum and the extent of damage to the pancreas.

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  • Reference Ranges:

    Serum
    Adult 13 – 60 U/L

    Serum
    Adult 13 – 60 U/L

  • Factors Affecting Result:

    Calcium complexing anticoagulants such as EDTA, citrate and fluoride should be avoided.

    In very rare cases, gammopathy, in particular IgM (Waldenstom's macroglobulinemia) may cause unreliable results.

    Calcium complexing anticoagulants such as EDTA, citrate and fluoride should be avoided.

    In very rare cases, gammopathy, in particular IgM (Waldenstom's macroglobulinemia) may cause unreliable results.

  • Routine Contact Name:

    Duty Biochemist

  • Routine Telephone:

    Freeman: 0191 244 8889 
    RVI: 0191 282 9719

    Freeman: 0191 244 8889 
    RVI: 0191 282 9719

  • Routine Email:

Availability:

All times

All sites

Turn Around:

- Urgent sample: 1 hour

Done on a single site. Where urgent samples come from a different site, they will be done within 3 hours.

- Routine sample: 24 hours

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