Lactate/pyruvate ratio, blood or CSF
Clinical Background:
Measurement of lactate and pyruvate is used in the investigation of lactic acidosis and can help with the diagnosis of pyruvate dehydrogenase (PDH) deficiency.
PDH deficiency is suspected when lact...
Measurement of lactate and pyruvate is used in the investigation of lactic acidosis and can help with the diagnosis of pyruvate dehydrogenase (PDH) deficiency.
PDH deficiency is suspected when lactate in blood or plasma > 5.0 mmol/L with lactate/pyruvate ratio < 25.
Pyruvate is very unstable in blood or CSF so samples must be taken into a tube containing perchloric acid (PCA) according to the protocol below.
Test Details
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Discipline:
Biochemistry
Biochemistry
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Specimen Container Adult:
Samples must be taken in a tube containing perchloric acid (PCA).
NuTH requests: contact the metabolic lab (or specimen reception out-of-hours) for a tube. Tubes are available on some GNCH wards. ...
Read MoreSamples must be taken in a tube containing perchloric acid (PCA).
NuTH requests: contact the metabolic lab (or specimen reception out-of-hours) for a tube. Tubes are available on some GNCH wards. They must be stored upright at 4oC.
Ensure patient details are hand-written on the tube. Do not attach a label to the tube.
External laboratories: contact the metabolic laboratory for a sample collection protocol.
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Specimen Container Paediatric:
Samples must be taken in a tube containing perchloric acid (PCA).
NuTH requests: contact the metabolic lab (or specimen reception out-of-hours) for a tube. Tubes are available on some GNCH wards. ...
Read MoreSamples must be taken in a tube containing perchloric acid (PCA).
NuTH requests: contact the metabolic lab (or specimen reception out-of-hours) for a tube. Tubes are available on some GNCH wards. They must be stored upright at 4oC.
Ensure patient details are hand-written on the tube. Do not attach a label to the tube.
External laboratories: contact the metabolic laboratory for a sample collection protocol.
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Minimum Volume Adult:
0.5 – 1.0 mL
0.5 – 1.0 mL
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Minimum Volume Paediatric:
0.5 – 1.0 mL
0.5 – 1.0 mL
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Transport Requirements:
NuTH requests: put the tube in a plastic bag to keep it dry, place on ice and transport to the Blood Sciences laboratory immediately.
External laboratories: freeze sample on receipt in the laborato...
Read MoreNuTH requests: put the tube in a plastic bag to keep it dry, place on ice and transport to the Blood Sciences laboratory immediately.
External laboratories: freeze sample on receipt in the laboratory and send frozen to the metabolic laboratory at the RVI.
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Reference Ranges:
CSF lactate 0 - 1.8 mmol/L
CSF pyruvate 0.04 – 0.15 mmol/L
CSF lactate/pyruvate ratio 0 – 18
Blood lactate ...
Read MoreCSF lactate 0 - 1.8 mmol/L
CSF pyruvate 0.04 – 0.15 mmol/L
CSF lactate/pyruvate ratio 0 – 18
Blood lactate 0 – 1.8 mmol/L
Blood pyruvate 0.035 – 0.150 mmol/L
Blood lactate/pyruvate ratio 0 – 18
Results are reported with interpretative comment.
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Routine Contact Name:
Duty Biochemist
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Routine Telephone:
Freeman: 0191 244 8889
RVI: 0191 282 9719
Freeman: 0191 244 8889
RVI: 0191 282 9719
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Routine Email:
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Specialist Test:
Yes
Yes
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Specialist Contact Name:
Metabolic Clinical Scientist
Metabolic Clinical Scientist
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Specialist Telephone:
0191 282 9685 (Consultant Clinical Scientist)
0191 282 0334 (Metabolic Laboratory)
0191 282 9685 (Consultant Clinical Scientist)
0191 282 0334 (Metabolic Laboratory)
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Specialist Email: