Newcastle Laboratories

Intermediary metabolites

Clinical Background:

A panel of tests including glucose, lactate, 3-OH butyrate and non-esterified fatty acids (NEFA) for the investigation of hypoglycaemia.

Analysis of intermediary metabolites in blood samples taken ...

A panel of tests including glucose, lactate, 3-OH butyrate and non-esterified fatty acids (NEFA) for the investigation of hypoglycaemia.

Analysis of intermediary metabolites in blood samples taken when the patient is hypoglycaemic (glucose <2.6 mmol/L) is used to help identify the cause.

See the UK Metabolic Biochemistry Network best practice guideline for investigation of hypoglycaemia in infants and children for further information on interpretation of results https://metbio.net/

See separate entries for plasma glucose and lactate

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Test Details

  • Discipline:

    Biochemistry

    Biochemistry

  • Specimen Container Adult:

    K-EDTA plasma (3-OH butyrate, NEFA).  

    Alternatively, a blood sample may be collected in a tube containing perchloric acid (PCA) and is suitable for analysis of glucose, lactate and 3-OH butyrate. ...

    K-EDTA plasma (3-OH butyrate, NEFA).  

    Alternatively, a blood sample may be collected in a tube containing perchloric acid (PCA) and is suitable for analysis of glucose, lactate and 3-OH butyrate.  This sample is not suitable for analysis of NEFA.

    Read More
  • Specimen Container Paediatric:

    K-EDTA plasma (3-OH butyrate, NEFA). 

    Alternatively, a blood sample may be collected in a tube containing perchloric acid (PCA) and is suitable for analysis of glucose, lactate and 3-OH butyrate.  ...

    K-EDTA plasma (3-OH butyrate, NEFA). 

    Alternatively, a blood sample may be collected in a tube containing perchloric acid (PCA) and is suitable for analysis of glucose, lactate and 3-OH butyrate.  This sample is not suitable for analysis of NEFA.

    Read More
  • Minimum Volume Adult:

    0.5 mL blood 

    0.5 mL blood 

  • Minimum Volume Paediatric:

    0.5 mL blood

    0.5 mL blood

  • Transport Requirements:

    NuTH requests: send to laboratory at ambient temperature

    External laboratories: send frozen

    NuTH requests: send to laboratory at ambient temperature

    External laboratories: send frozen

  • Reference Ranges:

    Reports are issued with interpretative comments

    Reports are issued with interpretative comments

  • Routine Contact Name:

    Duty Biochemist

  • Routine Telephone:

    Freeman: 0191 282 9719

    RVI: 0191 282 9719

    Freeman: 0191 282 9719

    RVI: 0191 282 9719

  • Routine Email:

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Metabolic Clinical Scientist

    Metabolic Clinical Scientist

  • Specialist Telephone:

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

  • Specialist Email:

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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