Newcastle Laboratories

Infectious Mononucleosis Screening

Clinical Background:

Infectious Mononucleosis (also known as Glandular Fever) is a widespread viral disease caused by the Epstein-Barr Virus (EBV), it is primarliy a disease of adolescents and young adults as >90% adul...

Infectious Mononucleosis (also known as Glandular Fever) is a widespread viral disease caused by the Epstein-Barr Virus (EBV), it is primarliy a disease of adolescents and young adults as >90% adults have acquired immunity by the age of 40. When exposed as children most people only develop flu-like symptoms. In the adolescents and young adults the disease is characterised by fever, sore throat, fatigue and swollen lymph nodes. The screening test uses a card system in which a drop of blood

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Test Details

  • Discipline:

    Haematology

    Haematology

  • Specimen Container Adult:

    Purple - EDTA

    Purple - EDTA

  • Specimen Container Paediatric:

    <p>Light Pink - EDTA</p>

    <p>Light Pink - EDTA</p>

  • Minimum Volume Adult:

    1.0mL

    1.0mL

  • Minimum Volume Paediatric:

    0.5mL

    0.5mL

  • Special Requirement:

    Ensure adequate mixing.

    Ensure adequate mixing.

  • Sample Stability:

    24 hrs

    24 hrs

  • Transport Requirements:

    Samples should be transported to lab without delay

    Samples should be transported to lab without delay

  • Freq Analysis:

    On request

    On request

  • Add On Test:

  • Quality Assurance:

    UK NEQAS Haematology

    UK NEQAS Haematology

  • Reference Ranges:

    See specific tests and overall morphology report in conjunction with result of slide test.

    See specific tests and overall morphology report in conjunction with result of slide test.

  • Factors Affecting Result:

    Infectious Mononucleosis Slide Test (Clearview IM II)

     

    Limitations of Test Procedure

     

    The Clearview IM II detects IM heterophile antibodies associated with EBV infection. These are present in 80-...

    Infectious Mononucleosis Slide Test (Clearview IM II)

     

    Limitations of Test Procedure

     

    The Clearview IM II detects IM heterophile antibodies associated with EBV infection. These are present in 80-90% of acute IM infections and can be detected in 60-70% of patients during the first week of clinical illness.

     

    Negative results can be obtained when insufficient levels of antibody are present. Where clinically indicated, or other laboratory results suggest, a repeat test should be performed on negative samples. A NEGATIVE result does not at any time preclude the possibility of IM infection.

     

    If the test is negative and clinical symptoms persist, additional testing using other clinical methods is recommended.

     

    The Clearview IM II is a qualitative in vitro diagnostic test. Neither the quantitative value nor the rate of increase in the IM antibody concentration can be determined by this qualitative test.

     

    Heterophile antibodies can also be demonstrated in rheumatoid arthritis, viral hepatitis, leukaemia and Burkett’s lymphoma.

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  • Other Info:

    Screening procedure for infectious mononucleosis involving:

    Full Blood Count

    Blood film review

    Infectious Mononucleosis slide test

    Screening procedure for infectious mononucleosis involving:

    Full Blood Count

    Blood film review

    Infectious Mononucleosis slide test

  • Routine Contact Name:

    RVI or Freeman Section Leaders

  • Routine Telephone:

    RVI: 0191 2820332, Freeman: 0191 2231195

    RVI: 0191 2820332, Freeman: 0191 2231195

  • Specialist Test:

    No

    No

  • Specialist Contact Name:

    Haematology and Haemostasis Healthcare Scientist

    Haematology and Haemostasis Healthcare Scientist

  • Specialist Telephone:

    0191 2139758

    0191 2139758

  • Specialist Email:

    alison.brown93@nhs.net

    alison.brown93@nhs.net

Availability:

All times, both sites

Turn Around:

Usually within 3 hours.

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