Newcastle Laboratories

Factor VIII Assay

Clinical Background:

Factor VIII (also known as Anti-Haemophilic Factor (AHF)) is a non-vitamin K sensitive coagulation protein, synthesised primarily in the liver, but also in endothelial cells throughout the body. Th...

Factor VIII (also known as Anti-Haemophilic Factor (AHF)) is a non-vitamin K sensitive coagulation protein, synthesised primarily in the liver, but also in endothelial cells throughout the body. The gene for factor VIII is on the X chromosome and the disorder deficiency produces (Haemophilia A) is a recessive X-linked bleeding disorder. Factor VIII is a co-factor with Factor IX in the generation of Factor Xa. The PT is normal and the APTT is prolonged in cases of Factor VIII deficiency. Low levels are also seen in von Willebrand disease as the von Willeberand Factor acts as a carrier/protector protein for Factor VIII and a reduction in vWF results in a greatly reduced half life of Factor VIII

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Test Details

  • Discipline:

    Haemostasis

    Haemostasis

  • Specimen Container Adult:

    Light blue - sodium citrate

    Light blue - sodium citrate

  • Specimen Container Paediatric:

    Light blue - sodium citrate

    Light blue - sodium citrate

  • Minimum Volume Adult:

    2.7mL

    2.7mL

  • Minimum Volume Paediatric:

    1.3mL

    1.3mL

  • Special Requirement:

    Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

    Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

  • Sample Stability:

    4 hrs

    4 hrs

  • Transport Requirements:

    Samples should be transported to lab without delay

    Samples should be transported to lab without delay

  • Freq Analysis:

    As required or weekly

    As required or weekly

  • Add On Test:

    All urgent add ons via telephone on 0191 28 24718, and must be confirmed via email to the appropriate email address

    tnu-tr.bloodsciencesadditions@nhs.net (internal)

    tnu-tr.bloodsciencesadditions@nh...

    All urgent add ons via telephone on 0191 28 24718, and must be confirmed via email to the appropriate email address

    tnu-tr.bloodsciencesadditions@nhs.net (internal)

    tnu-tr.bloodsciencesadditions@nhs.net (external)

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  • Quality Assurance:

    UK NEQAS Blood Coagulation

    UK NEQAS Blood Coagulation

  • Reference Ranges:

    50 - 150%

    50 - 150%

  • Other Info:

    A quantitative assay of coagulation factor VIII.

    A quantitative assay of coagulation factor VIII.

  • Routine Contact Name:

    Peter Strong

  • Routine Telephone:

    0191 282 0332

    0191 282 0332

  • Routine Email:

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Haematology/Haemostasis Health care Scientist

    Haematology/Haemostasis Health care Scientist

  • Specialist Telephone:

    0191 213 9758

    0191 213 9758

Availability:

Discuss with laboratory.

Out of hours discuss with on call Specialist Registrar.

Turn Around:

4 days

Urgent on request

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