Factor VIII Assay
Clinical Background:
Factor VIII (also known as Anti-Haemophilic Factor (AHF)) is a non-vitamin K sensitive coagulation protein, synthesised primarily in the liver, but also in endothelial cells throughout the body. Th...
Factor VIII (also known as Anti-Haemophilic Factor (AHF)) is a non-vitamin K sensitive coagulation protein, synthesised primarily in the liver, but also in endothelial cells throughout the body. The gene for factor VIII is on the X chromosome and the disorder deficiency produces (Haemophilia A) is a recessive X-linked bleeding disorder. Factor VIII is a co-factor with Factor IX in the generation of Factor Xa. The PT is normal and the APTT is prolonged in cases of Factor VIII deficiency. Low levels are also seen in von Willebrand disease as the von Willeberand Factor acts as a carrier/protector protein for Factor VIII and a reduction in vWF results in a greatly reduced half life of Factor VIII
Test Details
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Discipline:
Haemostasis
Haemostasis
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Specimen Container Adult:
Light blue - sodium citrate
Light blue - sodium citrate
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Specimen Container Paediatric:
Light blue - sodium citrate
Light blue - sodium citrate
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Minimum Volume Adult:
2.7mL
2.7mL
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Minimum Volume Paediatric:
1.3mL
1.3mL
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Special Requirement:
Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.
Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.
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Sample Stability:
4 hrs
4 hrs
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Transport Requirements:
Samples should be transported to lab without delay
Samples should be transported to lab without delay
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Freq Analysis:
As required or weekly
As required or weekly
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Add On Test:
tnu-tr.bloodsciencesadditions@nhs.net (external)
tnu-tr.bloodsciencesadditions@nhs.net (external)
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Quality Assurance:
UK NEQAS Blood Coagulation
UK NEQAS Blood Coagulation
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Reference Ranges:
50 - 150IU/dL
Note: For Haemostatic tests, paediatric reference ranges may be different to adult ranges. Please see
https://www.newcastlelaboratories.com/lab_service/core-247/
for details.
50 - 150IU/dL
Note: For Haemostatic tests, paediatric reference ranges may be different to adult ranges. Please see
https://www.newcastlelaboratories.com/lab_service/core-247/
for details.
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Other Info:
A quantitative assay of coagulation factor VIII.
A quantitative assay of coagulation factor VIII.
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Routine Contact Name:
RVI or Freeman Section Leaders
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Routine Telephone:
RVI: 0191 2820332, Freeman: 0191 2231195
RVI: 0191 2820332, Freeman: 0191 2231195
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Specialist Test:
Yes
Yes
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Specialist Contact Name:
Haematology and Haemostasis Healthcare Scientist
Haematology and Haemostasis Healthcare Scientist
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Specialist Telephone:
0191 2139758
0191 2139758
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Specialist Email:
alison.brown93@nhs.net
alison.brown93@nhs.net