Extractable Nuclear Antigen (ENA) Antibodies
Clinical Background:
Requests for ENA will be tested using multiplex technology on the BioPlex 2200 for the following antibodies, which are reported as Negative or Positive: Ro52 and Ro60 antibodies (speckled ANA patte...
Requests for ENA will be tested using multiplex technology on the BioPlex 2200 for the following antibodies, which are reported as Negative or Positive: Ro52 and Ro60 antibodies (speckled ANA pattern) – associated with Sjogren’s, SLE, Cutaneous lupus, neonatal lupus and congenital complete heart block. La antibodies (speckled ANA pattern) – associated with Sjogren’s, SLE and neonatal lupus. Centromere B Antibodies - found in 70-80% of cases of limited cutaneous scleroderma (CREST syndrome). Sm antibodies (speckled ANA pattern) – specific for SLE, common only in West Indians. SmRNP and RNP 68 antibodies (speckled ANA pattern) – associated with undifferentiated connective tissue disease (UCTD) and SLE. In SLE RNP antibodies occur with ds-DNA antibodies; in UCTD only RNP antibodies detected. Scl-70 antibodies (speckled ANA pattern) - associated with diffuse cutaneous scleroderma, especially in those patients with severe skin disease, musculoskeletal and cardiopulmonary disease. Jo-1 antibodies (ANA staining may not be seen, cytoplasmic staining may occur) - found in 25% of adult patients with autoimmune myositis and in 68% patients with anti-synthetase syndrome (myositis, arthritis, raynauds, interstitial lung disease). Results for anti-double-stranded DNA antibodies, Chromatin antibodies and Robosomal P protein antibodies are also produced when this method is used and these results will be given alongside those quoted above. PM-Scl antibodies (nucleolar ANA pattern) associated with polymyositis scleroderma overlap syndromes. Needs to be requested separately and is sent to an external laboratory for testing. Other specificities may be available through referral laboratories after discussion with Immunology Clinical staff.
Test Details
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Discipline:
Immunology
Immunology
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Specimen Container Adult:
Serum-SST
Serum-SST
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Specimen Container Paediatric:
Serum-Plain tube
Serum-Plain tube
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Minimum Volume Adult:
1 ml
1 ml
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Minimum Volume Paediatric:
0.5 ml
0.5 ml
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Special Requirement:
None
None
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Freq Analysis:
Daily
Daily
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Add On Test:
All urgent add ons via telephone on 0191 2821890, and must be confirmed via email to
All urgent add ons via telephone on 0191 2821890, and must be confirmed via email to
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Quality Assurance:
UK NEQAS for Antibodies to Nuclear and Related Antigens
UK NEQAS for Antibodies to Nuclear and Related Antigens
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Interpretation:
None
None
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Reference Ranges:
For all the listed antibodies; Normal Range: Negative
For all the listed antibodies; Normal Range: Negative
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Other Info:
Reported as Negative or Positive
Reported as Negative or Positive
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Routine Contact Name:
Autoimmunity Lab
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Routine Telephone:
0191 282 1890
0191 282 1890
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Routine Email: