CH50 and AP100
Clinical Background:
The CH50 and AP100 assays are ran conjunctively and test complement function
The use of these assays should be limited to the investigation of suspected complement deficiency or the monitoring of c...
The CH50 and AP100 assays are ran conjunctively and test complement function
The use of these assays should be limited to the investigation of suspected complement deficiency or the monitoring of complement blocking medications.
Any patient with recurrent pyogenic infections or meningococcal disease should be screened, as there is a possibility of a complement deficiency. Patients with atypical lupus may rarely show a defect in CH50 and complement components C1 or C2.
Test Details
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Discipline:
Immunology
Immunology
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Specimen Container Adult:
Serum-SST
Serum-SST
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Specimen Container Paediatric:
Serum-Plain tube
Serum-Plain tube
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Minimum Volume Adult:
2 ml
2 ml
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Minimum Volume Paediatric:
2 ml
2 ml
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Special Requirement:
Samples MUST be separated within 4 hours of venesection. Please ensure that the sample arrives in the laboratory as soon as possible after being taken.
Samples MUST be separated within 4 hours of venesection. Please ensure that the sample arrives in the laboratory as soon as possible after being taken.
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Freq Analysis:
Fortnightly
Fortnightly
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Add On Test:
All urgent add ons via telephone on 0191 282 4766, and must be confirmed via email to the appropriate email address
tnu-tr.bloodsciencesadditions@nhs.net
All urgent add ons via telephone on 0191 282 4766, and must be confirmed via email to the appropriate email address
tnu-tr.bloodsciencesadditions@nhs.net
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Interpretation:
Serial monitoring of the CH50 in SLE provides no additional information that cannot be obtained from C3 and C4 measurement and is therefore not recommended.
Serial monitoring of the CH50 in SLE provides no additional information that cannot be obtained from C3 and C4 measurement and is therefore not recommended.
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Reference Ranges:
AP100: Reported as either Normal or Absent.
CH50: 41.68-95.06 U/ml
AP100: Reported as either Normal or Absent.
CH50: 41.68-95.06 U/ml
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Other Info:
The AP100 assay is a lytic plate assay which is dependent on 100% haemolysis. The CH50 assay is a liposomal assay which uses a change in absorbance to determine complement activitySerial monitorin...
Read MoreThe AP100 assay is a lytic plate assay which is dependent on 100% haemolysis. The CH50 assay is a liposomal assay which uses a change in absorbance to determine complement activitySerial monitoring of the CH50 in SLE provides no additional information that cannot be obtained from C3 and C4 measurement and is therefore not recommended
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Routine Contact Name:
Proteins Lab
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Routine Telephone:
0191 282 4766
0191 282 4766
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Routine Email: