Newcastle Laboratories

B Cell Phenotyping

Clinical Background:

Use of B cell phenotyping in the investigation/monitoring of immunodeficiency should be discussed with the appropriate immunologist (adult or paediatric).

Use of B cell phenotyping in the investigation/monitoring of immunodeficiency should be discussed with the appropriate immunologist (adult or paediatric).

Test Details

  • Discipline:

    Immunology

    Immunology

  • Specimen Container Adult:

    Whole blood-EDTA

    Whole blood-EDTA

  • Specimen Container Paediatric:

    Whole blood-EDTA

    Whole blood-EDTA

  • Minimum Volume Adult:

    4 ml

    4 ml

  • Minimum Volume Paediatric:

    0.5 ml

    0.5 ml

  • Special Requirement:

    Note that samples should be received in the laboratory no later than 3pm on a Friday. Later arrivals will not be processed. Samples should be kept at room temperature.

    Note that samples should be received in the laboratory no later than 3pm on a Friday. Later arrivals will not be processed. Samples should be kept at room temperature.

  • Freq Analysis:

    Daily

    Daily

  • Add On Test:

    Use the contact details below to discuss if an add-on is required

    Use the contact details below to discuss if an add-on is required

  • Interpretation:

    Samples that are clotted or over 2 days old will not be processed. Test may not be possible on patients with low B cell numbers.

    Samples that are clotted or over 2 days old will not be processed. Test may not be possible on patients with low B cell numbers.

  • Reference Ranges:

    Not supplied

    Not supplied

  • Other Info:

    This test is usually done in conjunction with lymphocyte surface markers, in which case a separate sample is not required. The following markers are included: CD19, CD27 and IgD. Samples should ide...

    This test is usually done in conjunction with lymphocyte surface markers, in which case a separate sample is not required. The following markers are included: CD19, CD27 and IgD. Samples should ideally be tested as fresh as possible. Keep at room temperature at all times. Samples should arrive in the laboratory no later than 15:00 on a Friday. Samples arriving later than this will not be processed. Test may not be possible on patients with low B cell numbers.

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  • Routine Contact Name:

    Flow Cytometry Lab

  • Routine Telephone:

    0191 282 5078

    0191 282 5078

  • Routine Email:

Availability:

B cell phenotypes are run daily Monday to Friday 09:00-15:00

Turn Around:

1-2 routine working days

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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