Newcastle Laboratories

Anti-Neutrophil Cytoplasmic Antibodies (ANCA)

Clinical Background:

ANCA is a test for Granulomatosis with polyangiitis, microscopic polyangiitis and eosinophilic granulomatosis with polyangiitis and may appear as either perinuclear (P-ANCA) or cytoplasmic (C-ANCA)

ANCA is a test for Granulomatosis with polyangiitis, microscopic polyangiitis and eosinophilic granulomatosis with polyangiitis and may appear as either perinuclear (P-ANCA) or cytoplasmic (C-ANCA)

Test Details

  • Discipline:

    Immunology

    Immunology

  • Specimen Container Adult:

    Serum-SST

    Serum-SST

  • Specimen Container Paediatric:

    Serum-Plain tube

    Serum-Plain tube

  • Minimum Volume Adult:

    1 ml

    1 ml

  • Minimum Volume Paediatric:

    0.5 ml

    0.5 ml

  • Special Requirement:

    None

    None

  • Freq Analysis:

    Available on request

    Available on request

  • Add On Test:

    All urgent add ons via telephone on 0191 2821890, and must be confirmed via email to the appropriate email address

    nuth.autoimmunity@nhs.net

     

    All urgent add ons via telephone on 0191 2821890, and must be confirmed via email to the appropriate email address

    nuth.autoimmunity@nhs.net

     

  • Quality Assurance:

    UK NEQAS for Autoimmune Serology ANCA and GBM

    UK NEQAS for Autoimmune Serology ANCA and GBM

  • Interpretation:

    90% of patients with WG will be positive for C-ANCA. P-ANCA is less specific and can be found in several forms of vasculitis. Serial monitoring of ANCA may help in the prediction of relapses.

    90% of patients with WG will be positive for C-ANCA. P-ANCA is less specific and can be found in several forms of vasculitis. Serial monitoring of ANCA may help in the prediction of relapses.

  • Reference Ranges:

    Normal Range: Negative

    Normal Range: Negative

  • Other Info:

    Reported as Negative or a titration value for Positive samples.

    Reported as Negative or a titration value for Positive samples.

  • Routine Contact Name:

    Autoimmunity Lab

  • Routine Telephone:

    0191 282 1890

    0191 282 1890

  • Routine Email:

Availability:

Immunofluorescence ANCA is not routinely run, but may be specifically requested where the first-line tests for ANCA-related small vessel vasculitis – Myeloperoxidase (MPO) and Proteinase 3 (PR3) antibodies – are both Negative and there is still strong clinical suspicion of ANCA-related small vessel vasculitis.

Turn Around:

7 days

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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