Newcastle Laboratories

Antenatal & Postnatal Suitability

Clinical Background:

Prevention of Haemolytic Disease of the Fetus and Newborn (HDFN) in D Negative women to reduce the chances of forming immune anti-D antibodies (i.e. becoming sensitised).
Anti-D immunoglobulin prop...

Prevention of Haemolytic Disease of the Fetus and Newborn (HDFN) in D Negative women to reduce the chances of forming immune anti-D antibodies (i.e. becoming sensitised).
Anti-D immunoglobulin prophylaxis is given routinely at 28-30 weeks gestation, within 72 hours following any sensitising events (an incident in which a fetomaternal haemorrhage may have occurred) and within 72 hours of delivery of a D positive baby.

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Test Details

  • Discipline:

    Transfusion

    Transfusion

  • Specimen Container Adult:

    6mL K2 EDTA

    6mL K2 EDTA

  • Specimen Container Paediatric:

    6mL K2 EDTA

    6mL K2 EDTA

  • Minimum Volume Adult:

    6mL

    6mL

  • Minimum Volume Paediatric:

    2mL

    2mL

  • Special Requirement:

    Test can be ordered via EPR PowerChart and taken with the BloodTrack bedside sample labelling system.
    The Trust operates a zero tolerance policy. Deviation from Trust Sample Labelling Policy will re...

    Test can be ordered via EPR PowerChart and taken with the BloodTrack bedside sample labelling system.
    The Trust operates a zero tolerance policy. Deviation from Trust Sample Labelling Policy will result in the sample being rejected and a repeat requested.

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  • Sample Stability:

    Short term storage: 24 hours at room temperature.
    Long term storage: 7 days at 4-6°C

    Short term storage: 24 hours at room temperature.
    Long term storage: 7 days at 4-6°C

  • Transport Requirements:

    Sample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot...

    Sample should be transported to Laboratory Medicine Reception via GP courier, hospital air-tube system or hand delivered to maintain storage conditions. Samples must not be subjected to extreme hot or cold conditions prior to testing.

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  • Add On Test:

    Request for issue of anti-D must be discussed directly with the Transfusion laboratory.
    RVI: 0191 2824435

    Request for issue of anti-D must be discussed directly with the Transfusion laboratory.
    RVI: 0191 2824435

  • Interpretation:

    SUITABLE to receive prophylactic anti-D injection: Mother is D negative and DOES NOT have immune anti-D in her plasma after delivery of a D POSITIVE baby. Routine antenatal and postnatal prophylact...

    SUITABLE to receive prophylactic anti-D injection: Mother is D negative and DOES NOT have immune anti-D in her plasma after delivery of a D POSITIVE baby. Routine antenatal and postnatal prophylactic anti-D should always be given irrespective of additional anti-D immunoglobulin that may have been given due to a previous sensitising event. FMH estimation is performed to determine if any ADDITIONAL doses of anti-D prophylaxis are required; 1500IU Anti-D i.m is sufficient for up to 12mL FMH.

    NOT SUITABLE to receive prophylactic Anti-D injection: Mother is D positive Mother is D negative with known immune anti-D in her plasma after delivery of a D negative baby

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  • Factors Affecting Result:

    Anti-D immunoglobulin is not indicated: if the woman declines after discussion of the benefits and risks, if the woman has previously had cFFDNA testing for this particular pregnancy and the result...

    Anti-D immunoglobulin is not indicated: if the woman declines after discussion of the benefits and risks, if the woman has previously had cFFDNA testing for this particular pregnancy and the result states that the baby is D negative and chooses to decline with this information, if the woman is in labour, the patient is known to be sensitive to anti-D immunoglobulin or any of the products ingredients, the woman is D positive, the woman has immune anti-D.
    Please refer to antenatal clinic guidelines for management in this situation.
    For Suitability specimens: Insufficient, clotted or haemolysed specimens. Detection of previous prophylactic anti-D injections or other maternal atypical antibodies will require investigation and may extend turnaround times.

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  • Other Info:

    Rhophylac anti-D injections must be kept refrigerated at 4-6oC.

    Rhophylac anti-D injections must be kept refrigerated at 4-6oC.

  • Routine Contact Name:

    RVI Transfusion Section Leads

  • Routine Telephone:

    RVI: 0191 282 4335

    RVI: 0191 282 4335

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Transfusion Manager

    Transfusion Manager

  • Specialist Telephone:

    0192 213 9169

    0192 213 9169

Availability:

24/7

Turn Around:

Sensitising events: Issue of primary anti-D injection within 2 hours. Issue of subsequent anti-D injection is required within 24 hours.
Suitability and post natal anti-D injection: From 08:30 -20:00:- available within 6 hours (subject to the detection of an alloantibody or previous prophylaxis injection.)
From 20:00 -08:30: available by the end for the following morning.
Routine prophylaxis: By the date documented on the request form.

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