Activated Protein C Resistance
Clinical Background:
Activated Protein C resistance is a haemostatic disorder characterised by poor anticoagulant response to activated protein C (APC), this results in an increased thrombotic risk. It may be acquired ...
Activated Protein C resistance is a haemostatic disorder characterised by poor anticoagulant response to activated protein C (APC), this results in an increased thrombotic risk. It may be acquired or congenital. The best known and most common congenital form is due to a mutation in the Factor V molecule known as Factor V Leiden. The assay is a clot based screening test and if positive, a pcr is performed for the Factor V Leiden mutation. Factor V Leiden is inherited in an autosomal dominant manner and the thrombotic risk is increased in Heterozygotes and greatly increased in Homozygotes. Pregnancy and It is the most common inherited thrombotic risk factor with up to 5% of the population being Heterozygous. Oestrogen containing contraceptives or HRT are signifcant thrombotic risk factors for DVT and PE in individuals with Factor V Leiden mutation. This assay is part of the Thrombophilia screen
Test Details
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Discipline:
Haemostasis
Haemostasis
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Specimen Container Adult:
Light blue - sodium citrate
Light blue - sodium citrate
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Specimen Container Paediatric:
Light blue - sodium citrate
Light blue - sodium citrate
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Minimum Volume Adult:
10.8mL
10.8mL
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Minimum Volume Paediatric:
5.2mL
5.2mL
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Special Requirement:
Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.
Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.
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Sample Stability:
4 hrs
4 hrs
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Transport Requirements:
Samples should be transported to lab without delay
Samples should be transported to lab without delay
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Freq Analysis:
As required or weekly
As required or weekly
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Add On Test:
tnu-tr.bloodsciencesadditions@nhs.net (external)
tnu-tr.bloodsciencesadditions@nhs.net (external)
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Quality Assurance:
UK NEQAS Blood Coagulation
UK NEQAS Blood Coagulation
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Reference Ranges:
2.3 - 3.5
2.3 - 3.5
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Other Info:
Assessment of APC resistance ratio as part of a thrombophilia screen. Suggestive results will automatically be referred for Factor V Leiden analysis.
Assessment of APC resistance ratio as part of a thrombophilia screen. Suggestive results will automatically be referred for Factor V Leiden analysis.
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Routine Contact Name:
RVI or Freeman Section Leaders
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Routine Telephone:
RVI: 0191 2820332, Freeman: 0191 2231195
RVI: 0191 2820332, Freeman: 0191 2231195
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Routine Email:
Joanne.hall8@nhs.net
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Specialist Test:
Yes
Yes
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Specialist Contact Name:
Haematology and Haemostasis Healthcare Scientist
Haematology and Haemostasis Healthcare Scientist
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Specialist Telephone:
0191 2139758
0191 2139758
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Specialist Email:
alison.brown93@nhs.net
alison.brown93@nhs.net