Newcastle Laboratories

Free fatty acids (non-esterified fatty acids, NEFA), plasma

Clinical Background:

In normal subjects, circulating NEFA and ketone body concentrations rise simultaneously during fasting. Published data on normal fasting children suggests that log [NEFA] and log total [ketone bodi...

In normal subjects, circulating NEFA and ketone body concentrations rise simultaneously during fasting. Published data on normal fasting children suggests that log [NEFA] and log total [ketone bodies] are linearly related. A rise in the NEFA concentration out of proportion to the rise in ketone bodies is suggestive of a defect of b-oxidation or ketone body synthesis. During hypoglycaemia a low circulating NEFA and ketone bodies suggests hyperinsulinism.

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Test Details

  • Discipline:

    Biochemistry

    Biochemistry

  • Specimen Container Adult:

    EDTA plasma

    EDTA plasma

  • Specimen Container Paediatric:

    EDTA plasma

     

    EDTA plasma

     

  • Minimum Volume Adult:

    2 mL blood

    2 mL blood

  • Minimum Volume Paediatric:

    1 mL blood

    1 mL blood

  • Special Requirement:

    To investigate cause of hypoglycaemia, collect sample during a hypoglycaemic episode or following a monitored prolonged fast.

    Lithium heparin sample is NOT acceptable.

    To investigate cause of hypoglycaemia, collect sample during a hypoglycaemic episode or following a monitored prolonged fast.

    Lithium heparin sample is NOT acceptable.

  • Sample Stability:

    Unseparated whole blood 4 hours at 4°C

    Separated plasma or serum 2 months at -20°C

    Unseparated whole blood 4 hours at 4°C

    Separated plasma or serum 2 months at -20°C

  • Transport Requirements:

    Local (NuTH) requests:  Send samples to the laboratory at ambient temperature.  Samples should be forwarded to the RVI for processing.  N.B. Check if other tests (e.g. blood spot acylcarnitines) ar...

    Local (NuTH) requests:  Send samples to the laboratory at ambient temperature.  Samples should be forwarded to the RVI for processing.  N.B. Check if other tests (e.g. blood spot acylcarnitines) are required before processing the sample.

    External requests: send frozen plasma or serum to RVI.

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  • Reference Ranges:

    When analysed as part of an intermediary metabolite profile, results are reported with an interpretative comment.

    Reference ranges are otherwise as follows:

    Non-fasting: < 0.9 mmol/L

    Fasting (overa...

    When analysed as part of an intermediary metabolite profile, results are reported with an interpretative comment.

    Reference ranges are otherwise as follows:

    Non-fasting: < 0.9 mmol/L

    Fasting (overall range): 0.5 - 2.8 mmol/L

    15h fast: 0.5 - 1.6 mmol/L

    20h fast: 0.6 - 2.6 mmol/L

    24h fast: 1.0 - 2.8 mmol/L

     

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  • Factors Affecting Result:

    Heparin (stimulates lipoprotein lipase therefore NEFA release from triglycerides).
    Haemolysis

    Heparin (stimulates lipoprotein lipase therefore NEFA release from triglycerides).
    Haemolysis

  • Routine Contact Name:

    Duty biochemist

  • Routine Telephone:

    Freeman: 0191 244 8889

    RVI: 0191 282 9719

    Freeman: 0191 244 8889

    RVI: 0191 282 9719

  • Routine Email:

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Metabolic Clinical Scientist

    Metabolic Clinical Scientist

  • Specialist Telephone:

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

    0191 282 9685 (Consultant Clinical Scientist)

    0191 282 0334 (Metabolic Laboratory)

  • Specialist Email:

Availability:

Available during full access hours
Assayed weekly
Site of analysis: RVI

Turn Around:

Within 1 week

Send To:

Department of Blood Sciences

Level 3
Leazes Wing
Royal Victoria Infirmary
Queen Victoria Road
Newcastle upon Tyne
NE1 4LP

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