Immunophenotyping / Cell Markers
Clinical Background:
Immunophenotyping by flow cytometry is an essential component of the diagnostic work-up of many haematological malignancies including leukaemia, lymphoma and myeloma. This involves the use of fluor...
Immunophenotyping by flow cytometry is an essential component of the diagnostic work-up of many haematological malignancies including leukaemia, lymphoma and myeloma. This involves the use of fluorescently labelled antibodies that bind to antigens on the surface of white cells, which are then detected by the flow cytometer. The pattern of expression, along with morphology, is used to determine the cell linage and maturity, in many cases providing vital diagnostic information, from which genetic assays are performed to obtain further classification and prognostic information.
Requests for flow cytometry in the context of Haematological Malignancy should be requested via Northern England Haemato-Oncology Diagnostic Service (NEHODS): visit https://www.newcastlelaboratories.com/lab_service/nehods/ or contact NEHODS on 0191 2825028 or tnu-tr.nehods@nhs.net.
Test Details
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Discipline:
Flow lab
Flow lab
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Specimen Container Adult:
EDTA peripheral blood or bone marrow
EDTA peripheral blood or bone marrow
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Specimen Container Paediatric:
EDTA peripheral blood or bone marrow
EDTA peripheral blood or bone marrow
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Minimum Volume Adult:
Ideally >1.0mL
Ideally >1.0mL
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Minimum Volume Paediatric:
Ideally >1.0mL
Ideally >1.0mL
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Special Requirement:
Ensure adequate mixing.
Ensure adequate mixing.
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Add On Test:
Use the contact details below to discuss if an add-on is required
Use the contact details below to discuss if an add-on is required
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Interpretation:
All flow cytometry results are provided with a clinical interpretive report.
All flow cytometry results are provided with a clinical interpretive report.
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Reference Ranges:
See interpretative report accompanying marker results.
See interpretative report accompanying marker results.
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Other Info:
Discuss with laboratory to determine panel of markers required.
Discuss with laboratory to determine panel of markers required.
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Routine Contact Name:
Flow Cytometry Lab
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Routine Telephone:
0191 282 5078
0191 282 5078
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Routine Email: