Bone marrow trephine (NEHODS)
Clinical Background:
Requested on advice of consultant or specialist registrar in haematology. Primarily undertaken during investigation of abnormal FBC to look for evidence of haematological malignancy. Also used to i...
Requested on advice of consultant or specialist registrar in haematology. Primarily undertaken during investigation of abnormal FBC to look for evidence of haematological malignancy. Also used to investigate lymphadenopathy/splenomegaly, abnormal peripheral blood morphology, if there is a paraprotein, to stage haematological malignancies, look for causes of pyrexia of unknown origin or to look for causes of unexplained anaphylaxis. After processing and staining, reviewing the thin sections using a microscope allows the team to look at the structure of the material as well as the cellular content.
Test Details
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Discipline:
NEHODS
NEHODS
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Specimen Container Adult:
Formalin 4% (or paraffin block if processed locally)
Formalin 4% (or paraffin block if processed locally)
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Specimen Container Paediatric:
Formalin 4% (or paraffin block if processed locally)
Formalin 4% (or paraffin block if processed locally)
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Minimum Volume Adult:
N/A
N/A
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Minimum Volume Paediatric:
N/A
N/A
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Special Requirement:
Please complete NEHODS request form. If sending us a trephine paraffin block please also send us one H&E slide.
Please complete NEHODS request form. If sending us a trephine paraffin block please also send us one H&E slide.
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Sample Stability:
N/A
N/A
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Transport Requirements:
Samples should be sent to laboratory immediately.
Samples should be sent to laboratory immediately.
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Freq Analysis:
Daily
Daily
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Add On Test:
N/A
N/A
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Quality Assurance:
UK NEQAS trephine quality and interpretation scheme
UK NEQAS trephine quality and interpretation scheme
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Interpretation:
By specialist registrar or consultant haematologist or consultant histopathologist
By specialist registrar or consultant haematologist or consultant histopathologist
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Reference Ranges:
N/A
N/A
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Factors Affecting Result:
Quality and size of sample
Quality and size of sample
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Referenced Documents:
N/A
N/A
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Other Info:
H&E and reticulin will be made in all cases. Special stains may be required depending on morphological assessment. Common special stains include Giemsa PAS, Congo red, Sirius red, Masson trichrome,...
Read MoreH&E and reticulin will be made in all cases. Special stains may be required depending on morphological assessment. Common special stains include Giemsa PAS, Congo red, Sirius red, Masson trichrome, Alcian blue. Immunohistochemistry will be performed depending on morphological assessment and clinical details. A wide range of stains are available covering B-cell and T-cell lymphoma, myeloid neoplasms, histiocytosis and plasma cell neoplasms. When required genetic analysis e.g. FISH, SNP array, molecular testing will be requested on trephine blocks.