Newcastle Laboratories

Activated Protein C Resistance

Clinical Background:

Activated Protein C resistance is a haemostatic disorder characterised by poor anticoagulant response to activated protein C (APC), this results in an increased thrombotic risk. It may be acquired ...

Activated Protein C resistance is a haemostatic disorder characterised by poor anticoagulant response to activated protein C (APC), this results in an increased thrombotic risk. It may be acquired or congenital. The best known and most common congenital form is due to a mutation in the Factor V molecule known as Factor V Leiden. The assay is a clot based screening test and if positive, a pcr is performed for the Factor V Leiden mutation. Factor V Leiden is inherited in an autosomal dominant manner and the thrombotic risk is increased in Heterozygotes and greatly increased in Homozygotes. Pregnancy and It is the most common inherited thrombotic risk factor with up to 5% of the population being Heterozygous. Oestrogen containing contraceptives or HRT are signifcant thrombotic risk factors for DVT and PE in individuals with Factor V Leiden mutation. This assay is part of the Thrombophilia screen

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Test Details

  • Discipline:

    Haemostasis

    Haemostasis

  • Specimen Container Adult:

    Light blue - sodium citrate

    Light blue - sodium citrate

  • Specimen Container Paediatric:

    Light blue - sodium citrate

    Light blue - sodium citrate

  • Minimum Volume Adult:

    10.8mL

    10.8mL

  • Minimum Volume Paediatric:

    5.2mL

    5.2mL

  • Special Requirement:

    Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

    Tube must be correctly filled to line, to ensure correct ratio of blood to anticoagulant, ensure adequate mixing.

  • Sample Stability:

    4 hrs

    4 hrs

  • Transport Requirements:

    Samples should be transported to lab without delay

    Samples should be transported to lab without delay

  • Freq Analysis:

    As required or weekly

    As required or weekly

  • Add On Test:

    All urgent add ons via telephone on 0191 28 24718, and must be confirmed via email to the appropriate email address

    RVIadditions.Laboratory@nuth.nhs.uk (internal)

    tnu-tr.bloodsciencesadditions@nhs....

    All urgent add ons via telephone on 0191 28 24718, and must be confirmed via email to the appropriate email address

    RVIadditions.Laboratory@nuth.nhs.uk (internal)

    tnu-tr.bloodsciencesadditions@nhs.net (external)

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  • Quality Assurance:

    UK NEQAS Blood Coagulation

    UK NEQAS Blood Coagulation

  • Reference Ranges:

    3.4 - 6.0

    3.4 - 6.0

  • Other Info:

    Assessment of APC resistance ratio as part of a thrombophilia screen. Suggestive results will automatically be referred for Factor V Leiden analysis.

    Assessment of APC resistance ratio as part of a thrombophilia screen. Suggestive results will automatically be referred for Factor V Leiden analysis.

  • Routine Contact Name:

    Special Lab Freeman

  • Routine Telephone:

    0191 223 1780

    0191 223 1780

  • Routine Email:

    joanne.hall@nuth.nhs.uk

  • Specialist Test:

    Yes

    Yes

  • Specialist Contact Name:

    Haematology/Haemostasis Health Care Scientist

    Haematology/Haemostasis Health Care Scientist

  • Specialist Telephone:

    0191 213 9758

    0191 213 9758

Availability:

All testing performed at Freeman Haematology laboratory and available during routine hours, Monday to Friday. Testing is usually performed on a weekly basis, depending on batch size availability.

Turn Around:

2 weeks

Send To:

Department of Blood Sciences

Freeman Hospital
Freeman Road
Newcastle upon Tyne
NE7 7DN

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